The 15th Annual Course was held at Los Alamos National Flow Cytometry Resource (NFCR)
in New Mexico. All participants attended every lab. Special topic sessions ran evenings, 7–9 pm.
Lectures
- Introduction to flow cytometryLarry Seamer
- Fluorescence spectroscopy for flow cytometry usersLarry Sklar
- Probes for flow cytometryAlan Waggoner
- Flow cytometric data analysis: univariateBruce Bagwell
- Flow cytometric data analysis: multivariateGary Salzman
- DNA/cell proliferationHarry Crissman
- ApoptosisZbigniew Darzynkiewicz
- Flow cytometric immunophenotypingCarl Stewart
- Calcium transport and cell activationPeter Rabinovitch
- Chromosome analysisScott Cram
- Measurement of DNA content and intracellular antigensKen Bauer
- Analysis of leukemia and lymphomaGreg Stelzer
- Analysis of cellular adhesionYvan Rochon
- Special lecture — PCR analysis of flow-sorted chromosomesNigel Carter
Labs
All participants attended every lab.
- Build a flow cytometerJohn Martin, Jim Jett
- Data analysisGary Salzman, Bruce Bagwell
- Methods in immunophenotypingCarl & Sigie Stewart
- Chromosome sorting and FISHNigel Carter, Scott Cram, Babs Marrone
- Leukemia analysis — normal bone marrow / leukemia detectionGreg Stelzer
- Alternative methods of DNA and BrdUrd content analysisHarry Crissman
- Flow cytometric analysis of intracellular ionized calciumPeter Rabinovitch
Special Topics (evenings, 7–9 pm)
- New developments in flow cytometryJim Jett
- Phase sensitive flow cytometryJohn Steinkamp
- Kinetic cytometryRich Posner
- DNA fragment sizing by flow cytometryMitch Johnson
- DNA sequencing by flow cytometryDick Keller
- Multiparameter analysis of adipocyte differentiationMiriam Smyth
- Mutant cell selection by multi-cellular spheroid sortingJim Freyer
- Application of a new enzyme probe in studies of steroidogenesisBabs Marrone
- Optical phenotype analysis of lung cellsBruce Lehnert
